Analysis#

Pseudobulk.qc

Subsets each cell type to samples passing quality control (QC).

Pseudobulk.library_size

Calculate normalization factor-adjusted library sizes for each sample in each cell type, via the approach of edgeR's calcNormFactors().

Pseudobulk.CPM

Calculate counts per million for each cell type.

Pseudobulk.log_CPM

Calculate log counts per million for each cell type.

Pseudobulk.regress_out

Regress out covariates from obs.

Pseudobulk.DE

Perform differential expression (DE) on a Pseudobulk dataset with limma-voom.