Analysis#
Subsets each cell type to samples passing quality control (QC). |
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Calculate normalization factor-adjusted library sizes for each sample in each cell type, via the approach of edgeR's calcNormFactors(). |
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Calculate counts per million for each cell type. |
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Calculate log counts per million for each cell type. |
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Regress out covariates from obs. |
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Perform differential expression (DE) on a Pseudobulk dataset with limma-voom. |